Jump to main content
Jump to site search

Issue 11, 2014
Previous Article Next Article

A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells

Author affiliations

Abstract

Mesenchymal stem cells (MSCs) hold great promise in regenerative medicine due to their wide multilineage potential as well as their ability to suppress/modulate the immune response. Maintaining these cells in vitro and expanding them on a clinically relevant scale remains a challenge that needs to be addressed to realise their full potential. Current culture methods for MSCs typically rely on animal sourced substrates and often result in a heterogeneous population of cells with varying degrees of differentiation capacity. Here, a high-throughput platform was used to identify synthetic substrates for MSC culture that not only facilitated growth but also maintained the MSC phenotype. Two polymers, PU157 (synthesised from poly(butyleneglycol) and 4,4′-methylenediphenyldiisocyanate with 3-(dimethylamino)-1,2-propanediol as a chain extender) and PA338 (N-methylaniline modified poly(methylmethacrylate-co-glycidylmethacrylate)) were able to maintain the growth and phenotype of human embryonic derived mesenchymal progenitors (hES-MPs) and adipose derived MSCs (ADMSCs) for five and ten passages, respectively. Cell phenotype and multipotency were confirmed by flow cytometry analysis of ten MSC markers and differentiation analysis. These new polymer substrates provide a chemically defined synthetic surface for efficient, long-term MSC culture.

Graphical abstract: A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Apr 2014, accepted on 25 Jun 2014 and first published on 10 Jul 2014


Article type: Paper
DOI: 10.1039/C4BM00112E
Citation: Biomater. Sci., 2014,2, 1683-1692
  • Open access: Creative Commons BY license
  •   Request permissions

    A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells

    C. R. E. Duffy, R. Zhang, S. How, A. Lilienkampf, G. Tourniaire, W. Hu, C. C. West, P. de Sousa and M. Bradley, Biomater. Sci., 2014, 2, 1683
    DOI: 10.1039/C4BM00112E

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements