Issue 11, 2014

A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells

Abstract

Mesenchymal stem cells (MSCs) hold great promise in regenerative medicine due to their wide multilineage potential as well as their ability to suppress/modulate the immune response. Maintaining these cells in vitro and expanding them on a clinically relevant scale remains a challenge that needs to be addressed to realise their full potential. Current culture methods for MSCs typically rely on animal sourced substrates and often result in a heterogeneous population of cells with varying degrees of differentiation capacity. Here, a high-throughput platform was used to identify synthetic substrates for MSC culture that not only facilitated growth but also maintained the MSC phenotype. Two polymers, PU157 (synthesised from poly(butyleneglycol) and 4,4′-methylenediphenyldiisocyanate with 3-(dimethylamino)-1,2-propanediol as a chain extender) and PA338 (N-methylaniline modified poly(methylmethacrylate-co-glycidylmethacrylate)) were able to maintain the growth and phenotype of human embryonic derived mesenchymal progenitors (hES-MPs) and adipose derived MSCs (ADMSCs) for five and ten passages, respectively. Cell phenotype and multipotency were confirmed by flow cytometry analysis of ten MSC markers and differentiation analysis. These new polymer substrates provide a chemically defined synthetic surface for efficient, long-term MSC culture.

Graphical abstract: A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells

Supplementary files

Article information

Article type
Paper
Submitted
08 Apr 2014
Accepted
25 Jun 2014
First published
10 Jul 2014
This article is Open Access
Creative Commons BY license

Biomater. Sci., 2014,2, 1683-1692

Author version available

A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells

C. R. E. Duffy, R. Zhang, S. How, A. Lilienkampf, G. Tourniaire, W. Hu, C. C. West, P. de Sousa and M. Bradley, Biomater. Sci., 2014, 2, 1683 DOI: 10.1039/C4BM00112E

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