Issue 1, 2011

Arsenic speciation in wheat and wheat products using ultrasound- and microwave-assisted extraction and anion exchange chromatography-inductively coupled plasma mass spectrometry

Abstract

Wheat appears to be the major contributor to the intake of inorganic arsenic in countries where the diet is not rice-based. Ultrasound- and microwave-assisted extraction of arsenic in wheat and wheat based food using different solvents or enzymes was investigated in terms of extraction yield and species stability. Four extraction procedures were selected for the study of arsenic speciation in wheat and wheat products by anion exchange HPLC-ICP-MS using a PRP-X100 column with 10 mM NH4H2PO4, 10 mM NH4NO3, and 2% CH3OH at pH 5.5 as the mobile phase. Total arsenic in the samples ranged from 8.6 to 29.8 ng g−1 dry weight. About 95% of the arsenic was found to be present in inorganic form with AsIII as the most abundant species, whereas the remainder was mainly DMA. Microwave-assisted extraction with HNO3 was the most effective in liberating the arsenic species, which were then satisfactorily recovered from the chromatographic column. The LODs achieved, i.e., 0.35–0.46 ng g−1 dry weight, were suitable for the determination of arsenic species at the low levels found in sample extracts.

Graphical abstract: Arsenic speciation in wheat and wheat products using ultrasound- and microwave-assisted extraction and anion exchange chromatography-inductively coupled plasma mass spectrometry

  • This article is part of the themed collection: Speciation

Article information

Article type
Paper
Submitted
18 Aug 2010
Accepted
22 Nov 2010
First published
01 Dec 2010

J. Anal. At. Spectrom., 2011,26, 207-213

Arsenic speciation in wheat and wheat products using ultrasound- and microwave-assisted extraction and anion exchange chromatography-inductively coupled plasma mass spectrometry

M. D'Amato, F. Aureli, S. Ciardullo, A. Raggi and F. Cubadda, J. Anal. At. Spectrom., 2011, 26, 207 DOI: 10.1039/C0JA00125B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements