Jump to main content
Jump to site search

Issue 1, 2011
Previous Article Next Article

Ion mobility mass spectrometry: an elegant alternative focusing on speciation studies

Author affiliations

Abstract

This work is proposed to demonstrate the Traveling-Wave Ion Mobility Specrometry (TWIMS) coupled to Mass Spectrometry (MS) as an alternative technique for speciation analysis between metals/metalloids and biomolecules. Mobilities of bovine carbonic anhydrase bound to Ba2+, Cu2+, Pb2+, Zn2+, Cr3+, Cr6+, Se4+ and Se6+ were estimated. The metal belonging to the bovine carbonic anhydrase structure, commonly found in the commercially available enzyme, was removed by filtration, using centrifugal filter devices. Then, some metals/metalloids were added to 10.0 mmol L−1 ammonium acetate at pH = 6.8 enzyme solution. Experiments were carried out by direct insertion of the sample at 10 μL min−1 flow rate into the ESI source of the instrument. Carbonic anhydrase mobility varied according to the metal bound in its structure, following the order: Zn2+ < Cu2+ < Ba2+ < Pb2+. Metals with higher affinity by the enzyme, such as Zn2+ and Cu2+ had lower mobility, suggesting a higher structural modification, binding itself to the enzyme metallic site. Considering metals with different oxidation states, the enzyme mobility followed the order: Se4+ < Cr6+ < Se6+ < Cr3+.

Graphical abstract: Ion mobility mass spectrometry: an elegant alternative focusing on speciation studies

  • This article is part of the themed collection: Speciation
Back to tab navigation

Publication details

The article was received on 31 Aug 2010, accepted on 10 Nov 2010 and first published on 01 Dec 2010


Article type: Paper
DOI: 10.1039/C0JA00139B
Citation: J. Anal. At. Spectrom., 2011,26, 201-206
  •   Request permissions

    Ion mobility mass spectrometry: an elegant alternative focusing on speciation studies

    G. D. Souza Pessôa, E. J. Pilau, F. C. Gozzo and M. A. Zezzi Arruda, J. Anal. At. Spectrom., 2011, 26, 201
    DOI: 10.1039/C0JA00139B

Search articles by author

Spotlight

Advertisements