Pyridinum-based flexible tripodal cleft: a case of fluorescence sensing of ATP and dihydrogenphosphate under different conditions and cell imaging

Abstract

Pyridinium-based chemosensor 1 built on tris(aminomethyl)amine (tren) has been designed, synthesized and established as a chemosensor for ATP over ADP, AMP and a series of other anions in aqueous CH₃CN at pH 6.5. Compound 1 exhibits a significant change in emission upon complexation of ATP. In CH₃CN, the sensor selectively binds H₂PO₄⁻ and forms an excimer with significant intensity. Furthermore, the intracellular ATP detection using 1 was possible through fluorescent confocal imaging.