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Issue 6, 2016
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Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

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Abstract

A site-specific dual-color labeled ubiquitin for sensing deubiquitinase's activity was prepared by consecutively using chemoselective native chemical ligation reactions in a facile and efficient way. The prepared sensor was applied to establish a sensitive FRET-based assay for UCH-L3.

Graphical abstract: Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

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Publication details

The article was received on 03 Mar 2016, accepted on 13 Apr 2016 and first published on 13 Apr 2016


Article type: Communication
DOI: 10.1039/C6MB00165C
Citation: Mol. BioSyst., 2016,12, 1764-1767
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    Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

    L. Li, S. Zhang, Y. Li and Y. Chen, Mol. BioSyst., 2016, 12, 1764
    DOI: 10.1039/C6MB00165C

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