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Issue 6, 2016
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Sortase-mediated labelling of lipid nanodiscs for cellular tracing

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Abstract

Lipid nanodiscs have broad applications in membrane protein assays, biotechnology and materials science. Chemical modification of the nanodiscs to expand their functional attributes is generally desirable for all of these uses. We present a method for site-selective labelling of the N-terminus of the nanodisc's membrane scaffold protein (MSP) using the Sortase A protein. Labelling of the MSP was achieved when assembled within the lipid nanodisc architecture, demonstrating that this method can be used as a retrofit approach to modification of preformed nanodiscs before or during application. We label the MSP with a fluorescent fluorescein moiety and use them to image nanodisc uptake into HeLa cells. The Sortase A labelling method could be employed as a general approach to labelling nanodiscs with application-specific functionalities.

Graphical abstract: Sortase-mediated labelling of lipid nanodiscs for cellular tracing

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Publication details

The article was received on 17 Feb 2016, accepted on 04 Apr 2016 and first published on 06 Apr 2016


Article type: Communication
DOI: 10.1039/C6MB00126B
Citation: Mol. BioSyst., 2016,12, 1760-1763
  • Open access: Creative Commons BY license
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    Sortase-mediated labelling of lipid nanodiscs for cellular tracing

    A. I. Petrache, D. C. Machin, D. J. Williamson, M. E. Webb and P. A. Beales, Mol. BioSyst., 2016, 12, 1760
    DOI: 10.1039/C6MB00126B

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