Issue 2, 2012

A microfabricated micropillar liquid chromatographic chip monolithically integrated with an electrospray ionization tip

Abstract

We present the first monolithically integrated silicon/glass liquid chromatography-electrospray ionization microchip for mass spectrometry. The microchip is fabricated by bonding a silicon wafer, which has deep reactive ion etched micropillar-filled channels, together with a glass lid. Both the silicon channel and the glass lid have a through-wafer etched sharp tip that produces a stable electrospray. The microchip is also compatible with laser induced fluorescence (LIF) detection, due to the glass lid. Separation of drugs in less than 5 minutes using either SiO2 (normal phase) or C18 coated (reversed-phase) pillars with good sensitivity was demonstrated with mass spectrometric detection as well as separation of fluorescent compounds with LIF detection.

Graphical abstract: A microfabricated micropillar liquid chromatographic chip monolithically integrated with an electrospray ionization tip

Article information

Article type
Paper
Submitted
12 Sep 2011
Accepted
31 Oct 2011
First published
28 Nov 2011

Lab Chip, 2012,12, 325-332

A microfabricated micropillar liquid chromatographic chip monolithically integrated with an electrospray ionization tip

L. Sainiemi, T. Nissilä, R. Kostiainen, S. Franssila and R. A. Ketola, Lab Chip, 2012, 12, 325 DOI: 10.1039/C1LC20874H

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