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Issue 2, 2012
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An integrated chip for immunofluorescence and its application to analyze lysosomal storage disorders

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Abstract

Immunofluorescence (IF) is a common method to observe protein distribution and localization at the single-cell level through wide-field fluorescence or confocal microscopy. Conventional protocol for IF staining of cells typically requires a large amount of reagents, especially antibodies, and noticeable investment in both labor and time. Microfluidic technologies provide a cost-effective alternative: it can evaluate and optimize experimental conditions, and perform automatic and high-throughput IF staining on-chip. We employed this method to analyze lysosomal storage disorders (LSDs) based on the expression and morphological distribution of LAMP1 and LC3 in starving cells. With pneumatic valves integrated on-chip, the parallel staining process can be completed within a few hours. The total consumption of each antibody solution for the whole experiment is merely 0.3 μL. This device provides a promising tool for automated high-throughput molecular imaging at cell level that can be applied for diagnostic analysis.

Graphical abstract: An integrated chip for immunofluorescence and its application to analyze lysosomal storage disorders

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Publication details

The article was received on 04 Sep 2011, accepted on 21 Oct 2011 and first published on 28 Nov 2011


Article type: Paper
DOI: 10.1039/C1LC20845D
Citation: Lab Chip, 2012,12, 317-324
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    An integrated chip for immunofluorescence and its application to analyze lysosomal storage disorders

    J. Shen, Y. Zhou, T. Lu, J. Peng, Z. Lin, L. Huang, Y. Pang, L. Yu and Y. Huang, Lab Chip, 2012, 12, 317
    DOI: 10.1039/C1LC20845D

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