A novel photoswitchable enzyme cascade for powerful signal amplification in versatile bioassays

Abstract

This report outlines the construction of an advanced, exquisite photoswitchable enzyme cascade on the basis that tyrosinase (TYR) catalyzes the generation of dihydroxyphenylalanine (DOPA) coordinated TiO₂ nanoparticles (NPs) to form a light responsive nano-trigger that subsequently photoactivates the enzymatic activity of horseradish peroxidase (HRP). This photoswitchable enzyme cascade has a powerful signal transduction/amplification ability in TYR-based bioassays, and holds great promise to be applied in versatile applications.