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Issue 6, 2016
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Structure determination of microbial metabolites by the crystalline sponge method

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Abstract

The structures of metabolites produced in microgram quantities by enzymatic reductions with baker's yeast were analyzed using the crystalline sponge method. The X-ray data provided reliable structures for trace metabolites including their relative and absolute stereochemistries that are not fully addressed by conventional NMR and LC-MS analyses. Technically, combining two or more chromatographic purification techniques is essential because, unlike abundant synthetic compounds, extracted metabolites contain many low level UV-silent impurities. The crystalline sponge method coupled with HPLC purification (LC-SCD) would thus be a useful method for metabolic analysis and drug discovery.

Graphical abstract: Structure determination of microbial metabolites by the crystalline sponge method

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Publication details

The article was received on 06 Feb 2016, accepted on 23 Feb 2016 and first published on 24 Feb 2016


Article type: Edge Article
DOI: 10.1039/C6SC00594B
Citation: Chem. Sci., 2016,7, 3910-3913
  • Open access: Creative Commons BY license
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    Structure determination of microbial metabolites by the crystalline sponge method

    Y. Inokuma, T. Ukegawa, M. Hoshino and M. Fujita, Chem. Sci., 2016, 7, 3910
    DOI: 10.1039/C6SC00594B

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