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Issue 79, 2016
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A bioorthogonal approach for imaging the binding between Dasatinib and its target proteins inside living cells

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Abstract

Herein, we present a simple readout of the binding between a chemical drug and its target proteins in the cytoplasm by using a two-step bioorthogonal labeling method combined with spatially-localized expression of proteins. Dasatinib was modified with trans-cyclooctene (TCO), and its cytoplasmic target kinases were expressed in intracellular compartments, such as endosomes and F-actins. After bioorthogonal labeling, the colocalization between Dasatinib and its target proteins was observed in intracellular compartments.

Graphical abstract: A bioorthogonal approach for imaging the binding between Dasatinib and its target proteins inside living cells

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Publication details

The article was received on 26 Aug 2016, accepted on 05 Sep 2016 and first published on 05 Sep 2016


Article type: Communication
DOI: 10.1039/C6CC07011F
Citation: Chem. Commun., 2016,52, 11764-11767
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    A bioorthogonal approach for imaging the binding between Dasatinib and its target proteins inside living cells

    Y. Kim, Y. H. Kim, S. W. Kim, Y. J. Lee, D. Chae, K. Kim, Z. Lee, N. D. Kim, J. Choi, I. S. Choi and K. Lee, Chem. Commun., 2016, 52, 11764
    DOI: 10.1039/C6CC07011F

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