Jump to main content
Jump to site search

Issue 10, 2015
Previous Article Next Article

Unravelling the effect of temperature on viscosity-sensitive fluorescent molecular rotors

Author affiliations

Abstract

Viscosity and temperature variations in the microscopic world are of paramount importance for diffusion and reactions. Consequently, a plethora of fluorescent probes have evolved over the years to enable fluorescent imaging of both parameters in biological cells. However, the simultaneous effect of both temperature and viscosity on the photophysical behavior of fluorophores is rarely considered, yet unavoidable variations in temperature can lead to significant errors in the readout of viscosity and vice versa. Here we examine the effect of temperature on the photophysical behavior of three classes of viscosity-sensitive fluorophores termed ‘molecular rotors’. For each of the fluorophores we decouple the effect of temperature from the effect of viscosity. In the case of the conjugated porphyrin dimer, we demonstrate that, uniquely, simultaneous dual-mode lifetime and intensity measurements of this fluorophore can be used for measuring both viscosity and temperature concurrently.

Graphical abstract: Unravelling the effect of temperature on viscosity-sensitive fluorescent molecular rotors

Back to tab navigation

Supplementary files

Publication details

The article was received on 22 Jun 2015, accepted on 04 Jul 2015 and first published on 06 Jul 2015


Article type: Edge Article
DOI: 10.1039/C5SC02248G
Citation: Chem. Sci., 2015,6, 5773-5778
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Unravelling the effect of temperature on viscosity-sensitive fluorescent molecular rotors

    A. Vyšniauskas, M. Qurashi, N. Gallop, M. Balaz, H. L. Anderson and M. K. Kuimova, Chem. Sci., 2015, 6, 5773
    DOI: 10.1039/C5SC02248G

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements