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Issue 11, 2015
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Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents

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Abstract

Direct visualization of bioorthogonal alkyne or azide handles using fluorogenic azide–alkyne cycloaddition conducted on the surface of a blot membrane. The method eliminates the need for separation steps to remove excess small molecule reagents before attachment of antigen molecules or other visualization handles, and is especially useful for the analysis of peptides and small proteins. A variety of potential fluorogenic reagents are assessed, and sensitivity (<0.1 picomole) similar to current commercially available fluorescence imaging methods is possible.

Graphical abstract: Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents

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Publication details

The article was received on 29 Jul 2015, accepted on 20 Aug 2015 and first published on 24 Aug 2015


Article type: Communication
DOI: 10.1039/C5MB00510H
Citation: Mol. BioSyst., 2015,11, 2846-2849
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    Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents

    J. Ohata, F. Vohidov and Z. T. Ball, Mol. BioSyst., 2015, 11, 2846
    DOI: 10.1039/C5MB00510H

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