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Issue 14, 2015
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A microfluidic dual-well device for high-throughput single-cell capture and culture

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Abstract

In vitro culture of single cells facilitates biological studies by deconvoluting complications from cell population heterogeneity. However, there is still a lack of simple yet high-throughput methods to perform single cell culture experiments. In this paper, we report the development and application of a microfluidic device with a dual-well (DW) design concept for high-yield single-cell loading (~77%) in large microwells (285 and 485 μm in diameter) which allowed for cell spreading, proliferation and differentiation. The increased single-cell loading yield is achieved by using sets of small microwells termed “capture-wells” and big microwells termed “culture-wells” according to their utilities for single-cell capture and culture, respectively. This novel device architecture allows the size of the “culture” microwells to be flexibly adjusted without affecting the single-cell loading efficiency making it useful for cell culture applications as demonstrated by our experiments of KT98 mouse neural stem cell differentiation, A549 and MDA-MB-435 cancer cell proliferation, and single-cell colony formation assay with A549 cells in this paper.

Graphical abstract: A microfluidic dual-well device for high-throughput single-cell capture and culture

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Publication details

The article was received on 13 May 2015, accepted on 22 May 2015 and first published on 22 May 2015


Article type: Paper
DOI: 10.1039/C5LC00541H
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2015,15, 2928-2938
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    A microfluidic dual-well device for high-throughput single-cell capture and culture

    C. Lin, Y. Hsiao, H. Chang, C. Yeh, C. He, E. M. Salm, C. Chen, I. Chiu and C. Hsu, Lab Chip, 2015, 15, 2928
    DOI: 10.1039/C5LC00541H

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