Jump to main content
Jump to site search

Issue 31, 2014
Previous Article Next Article

Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?

Author affiliations

Abstract

Several laboratories had tested bioactivity of the materials in commercially available solution DMEM (Dulbecco’s Modified Eagle’s Medium) that is normally used for cultivation of cell cultures. The objective of this work was to find out whether it is possible to replace TRIS-buffered SBF currently used for bioactivity tests with the non-buffered DMEM solution. To understand the role of the organic part of the DMEM solution in the process of crystallization, we have prepared non-buffered solution simulating only its inorganic part (identified as I-solution). It was found that under static-dynamic test conditions calcite (CaCO3) and the amorphous phase of calcium phosphate (ACP) formed on the surface of the glass-ceramic (45S5 bioactive glass based) scaffold exposed to both solutions. Additionally, halite (NaCl) formed at the beginning of exposure to DMEM. Hydroxyapatite phase was not detected on the surface in either non-buffered solution. Organic components contained in the DMEM solution failed to prevent formation of crystalline phases. The present results indicate that it is not recommendable to use DMEM for bioactivity tests of glass-ceramic materials due to its low concentration of Ca2+ ions, high concentration of HCO3 ions and the necessity to maintain sterile environment during the test.

Graphical abstract: Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?

Back to tab navigation

Publication details

The article was received on 04 Feb 2014, accepted on 15 May 2014 and first published on 23 May 2014


Article type: Paper
DOI: 10.1039/C4TB00187G
Citation: J. Mater. Chem. B, 2014,2, 5068-5076
  • Open access: Creative Commons BY license
  •   Request permissions

    Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?

    D. Rohanová, A. R. Boccaccini, D. Horkavcová, P. Bozděchová, P. Bezdička and M. Častorálová, J. Mater. Chem. B, 2014, 2, 5068
    DOI: 10.1039/C4TB00187G

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements