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Issue 17, 2012
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X-Ray propagation imaging of a lipid bilayer in solution

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Abstract

We have used X-ray propagation imaging to visualize a less than 5 nm thick native lipid bilayer membrane freely suspended in aqueous solution. Contrast is formed by free space propagation of hard X-rays, with the membrane illuminated by a nano-focused, partially coherent synchrotron beam, at a controllable distance (defocus) behind the focal spot. Quantitative fitting of the magnified Fresnel fringes shows the transition from membranes swollen with solvent to the native bilayer, containing structural information at near-molecular resolution along the dimension perpendicular to the bilayer. We show first applications of this hybrid technique of propagation imaging and near-field diffraction to the investigation of ultra-thin organic films formed in micro-fluidic devices, namely the formation of a lipid bilayer by the adhesion of two constitutive monolayers.

Graphical abstract: X-Ray propagation imaging of a lipid bilayer in solution

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Publication details

The article was received on 05 Jan 2012, accepted on 14 Feb 2012 and first published on 13 Mar 2012


Article type: Paper
DOI: 10.1039/C2SM00032F
Citation: Soft Matter, 2012,8, 4595-4601
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    X-Ray propagation imaging of a lipid bilayer in solution

    A. Beerlink, S. Thutupalli, M. Mell, M. Bartels, P. Cloetens, S. Herminghaus and T. Salditt, Soft Matter, 2012, 8, 4595
    DOI: 10.1039/C2SM00032F

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