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Issue 17, 2012
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Fluorescence-based active site probes for profiling deubiquitinating enzymes

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Abstract

Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging.

Graphical abstract: Fluorescence-based active site probes for profiling deubiquitinating enzymes

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Publication details

The article was received on 03 Feb 2012, accepted on 07 Mar 2012 and first published on 28 Mar 2012


Article type: Communication
DOI: 10.1039/C2OB25258A
Citation: Org. Biomol. Chem., 2012,10, 3379-3383
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    Fluorescence-based active site probes for profiling deubiquitinating enzymes

    J. F. McGouran, H. B. Kramer, M. M. Mackeen, K. di Gleria, M. Altun and B. M. Kessler, Org. Biomol. Chem., 2012, 10, 3379
    DOI: 10.1039/C2OB25258A

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