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Issue 6, 2012
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Genome-scale genetic manipulation methods for exploring bacterial molecular biology

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Abstract

Bacteria are diverse and abundant, playing key roles in human health and disease, the environment, and biotechnology. Despite progress in genome sequencing and bioengineering, much remains unknown about the functional organization of prokaryotes. For instance, roughly a third of the protein-coding genes of the best-studied model bacterium, Escherichia coli, currently lack experimental annotations. Systems-level experimental approaches for investigating the functional associations of bacterial genes and genetic structures are essential for defining the fundamental molecular biology of microbes, preventing the spread of antibacterial resistance in the clinic, and driving the development of future biotechnological applications. This review highlights recently introduced large-scale genetic manipulation and screening procedures for the systematic exploration of bacterial gene functions, molecular relationships, and the global organization of bacteria at the gene, pathway, and genome levels.

Graphical abstract: Genome-scale genetic manipulation methods for exploring bacterial molecular biology

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Publication details

The article was received on 06 Feb 2012, accepted on 30 Mar 2012 and first published on 30 Mar 2012


Article type: Review Article
DOI: 10.1039/C2MB25040C
Citation: Mol. BioSyst., 2012,8, 1626-1638
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    Genome-scale genetic manipulation methods for exploring bacterial molecular biology

    A. Gagarinova and A. Emili, Mol. BioSyst., 2012, 8, 1626
    DOI: 10.1039/C2MB25040C

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