Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 6, 2012
Previous Article Next Article

Proteoglycan sequence

Author affiliations

Abstract

Proteoglycans (PGs) are among the most structurally complex biomacromolecules in nature. They are present in all animal cells and frequently exert their critical biological functions through interactions with protein ligands and receptors. PGs are comprised of a core protein to which one or multiple, heterogeneous, and polydisperse glycosaminoglycan (GAG) chains are attached. Proteins, including the protein core of PGs, are now routinely sequenced either directly using proteomics or indirectly using molecular biology through their encoding DNA. The sequencing of the GAG component of PGs poses a considerably more difficult challenge because of the relatively underdeveloped state of glycomics and because the control of their biosynthesis in the endoplasmic reticulum and the Golgi is poorly understood and not believed to be template driven. Recently, the GAG chain of the simplest PG has been suggested to have a defined sequence based on its top-down Fourier transform mass spectral sequencing. This review examines the advances made over the past decade in the sequencing of GAG chains and the challenges the field face in sequencing complex PGs having critical biological functions in developmental biology and pathogenesis.

Graphical abstract: Proteoglycan sequence

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 18 Jan 2012, accepted on 27 Mar 2012 and first published on 28 Mar 2012


Article type: Review Article
DOI: 10.1039/C2MB25021G
Citation: Mol. BioSyst., 2012,8, 1613-1625
  •   Request permissions

    Proteoglycan sequence

    L. Li, M. Ly and R. J. Linhardt, Mol. BioSyst., 2012, 8, 1613
    DOI: 10.1039/C2MB25021G

Search articles by author