Jump to main content
Jump to site search

Issue 22, 2012
Previous Article Next Article

Programmable microfluidic synthesis of spectrally encoded microspheres

Author affiliations

Abstract

Spectrally encoded fluorescent beads are an attractive platform for assay miniaturization and multiplexing in the biological sciences. Here, we synthesize hydrophilic PEG–acrylate polymer beads encoded with lanthanide nanophosphors using a fully automated microfluidic synthesis device. These beads are encoded by including varying amounts of two lanthanide nanophosphors relative to a third reference nanophosphor to generate 24 distinct ratios. These codes differ by less than 3% from their target values and can be distinguished from each other with an error rate of <0.1%. The encoded bead synthesis strategy we have used is readily extensible to larger numbers of codes, potentially up to millions, providing a new platform technology for assay multiplexing.

Graphical abstract: Programmable microfluidic synthesis of spectrally encoded microspheres

Back to tab navigation

Supplementary files

Publication details

The article was received on 20 Jun 2012, accepted on 21 Sep 2012 and first published on 25 Sep 2012


Article type: Paper
DOI: 10.1039/C2LC40699C
Citation: Lab Chip, 2012,12, 4716-4723
  • Open access:
  •   Request permissions

    Programmable microfluidic synthesis of spectrally encoded microspheres

    R. E. Gerver, R. Gómez-Sjöberg, B. C. Baxter, K. S. Thorn, P. M. Fordyce, C. A. Diaz-Botia, B. A. Helms and J. L. DeRisi, Lab Chip, 2012, 12, 4716
    DOI: 10.1039/C2LC40699C

Search articles by author

Spotlight

Advertisements