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Issue 14, 2012
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Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review

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Abstract

Nucleic Acid Testing (NAT) promises rapid, sensitive and specific diagnosis of infectious, inherited and genetic disease. The next generation of diagnostic devices will interrogate the genetic determinants of such conditions at the point-of-care, affording clinicians prompt reliable diagnosis from which to guide more effective treatment. The complex biochemical nature of clinical samples, the low abundance of nucleic acid targets in the majority of clinical samples and existing biosensor technology indicate that some form of nucleic acid amplification will be required to obtain clinically relevant sensitivities from the small samples used in point-of-care testing (POCT). This publication provides an overview and thorough review of existing technologies for nucleic acid amplification. The different methods are compared and their suitability for POCT adaptation are discussed. Current commercial products employing isothermal amplification strategies are also investigated. In conclusion we identify the factors impeding the integration of the methods discussed in fully automated, sample-to-answer POCT devices.

Graphical abstract: Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review

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Publication details

The article was received on 27 Jan 2012, accepted on 26 Mar 2012 and first published on 27 Mar 2012


Article type: Critical Review
DOI: 10.1039/C2LC40100B
Citation: Lab Chip, 2012,12, 2469-2486
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    Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review

    P. Craw and W. Balachandran, Lab Chip, 2012, 12, 2469
    DOI: 10.1039/C2LC40100B

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