Jump to main content
Jump to site search

Issue 22, 2012
Previous Article Next Article

Capture, isolation and release of cancer cells with aptamer-functionalized glass bead array

Author affiliations

Abstract

Early detection and isolation of circulating tumor cells (CTC) can enable better prognosis for cancer patients. A Hele-Shaw device with aptamer functionalized glass beads is designed, modeled, and fabricated to efficiently isolate cancer cells from a cellular mixture. The glass beads are functionalized with anti-epidermal growth factor receptor (EGFR) aptamer and sit in ordered array of pits in polydimethylsiloxane (PDMS) channel. A PDMS encapsulation is then used to cover the channel and to flow through cell solution. The beads capture cancer cells from flowing solution depicting high selectivity. The cell-bound glass beads are then re-suspended from the device surface followed by the release of 92% cells from glass beads using combination of soft shaking and anti-sense RNA. This approach ensures that the cells remain in native state and undisturbed during capture, isolation and elution for post-analysis. The use of highly selective anti-EGFR aptamer with the glass beads in an array and subsequent release of cells with antisense molecules provide multiple levels of binding and release opportunities that can help in defining new classes of CTC enumeration devices.

Graphical abstract: Capture, isolation and release of cancer cells with aptamer-functionalized glass bead array

Back to tab navigation

Supplementary files

Publication details

The article was received on 15 Dec 2011, accepted on 17 Jul 2012 and first published on 27 Jul 2012


Article type: Paper
DOI: 10.1039/C2LC21251J
Citation: Lab Chip, 2012,12, 4693-4701
  •   Request permissions

    Capture, isolation and release of cancer cells with aptamer-functionalized glass bead array

    Y. Wan, Y. Liu, P. B. Allen, W. Asghar, M. A. I. Mahmood, J. Tan, H. Duhon, Y. Kim, A. D. Ellington and S. M. Iqbal, Lab Chip, 2012, 12, 4693
    DOI: 10.1039/C2LC21251J

Search articles by author

Spotlight

Advertisements