Jump to main content
Jump to site search

Issue 3, 2012
Previous Article Next Article

Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium

Author affiliations

Abstract

We present a microfluidic epithelial wound-healing assay that allows characterization of the effect of hepatocyte growth factor (HGF) on the regeneration of alveolar epithelium using a flow-focusing technique to create a regular wound in the epithelial monolayer. The phenotype of the epithelial cell was characterized using immunostaining for tight junction (TJ) proteins and transmission electron micrographs (TEMs) of cells cultured in the microfluidic system, a technique that is reported here for the first time. We demonstrate that alveolar epithelial cells cultured in a microfluidic environment preserve their phenotype before and after wounding. In addition, we report a wound-healing benefit induced by addition of HGF to the cell culture medium (19.2 vs. 13.5 μm h−1 healing rate).

Graphical abstract: Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Sep 2011, accepted on 15 Nov 2011 and first published on 06 Dec 2011


Article type: Paper
DOI: 10.1039/C1LC20879A
Citation: Lab Chip, 2012,12, 640-646
  •   Request permissions

    Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium

    M. Felder, P. Sallin, L. Barbe, B. Haenni, A. Gazdhar, T. Geiser and O. Guenat, Lab Chip, 2012, 12, 640
    DOI: 10.1039/C1LC20879A

Search articles by author

Spotlight

Advertisements