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Issue 3, 2012
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A multi-mode fiber probe for holographic micromanipulation and microscopy

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Abstract

Holographic tweezers have revolutionized the way we do experiments at the micron scale. The possibility of applying controlled force fields on simultaneously trapped micro-particles has allowed to directly probe interactions and mechanical properties of colloids, macromolecules and living cells. Holographic micromanipulation requires the careful shaping of a laser beam that is then focused by a microscope objective onto a micro-hologram in the sample volume. The same objective is used for imaging. That approach is therefore limited to in vitro samples contained in transparent cells that are easily accessed optically. Here we demonstrate that the complex light propagator of a real multimode fiber can be directly measured. That allows to transmit a micro-hologram through a 1 metre long (60 μm core) optical fiber and produce dynamic arrays of focused spots at the fiber output. We show that those spots can be used for interactive holographic micromanipulation of micron sized beads facing the fiber tip. Scanning a single spot across the output fiber we can employ the same fiber as a probe for scanning fluorescence microscopy. Our findings open the way towards the fabrication of endoscopic probes which could be capable of seeing and manipulating single cells deep into biological tissues.

Graphical abstract: A multi-mode fiber probe for holographic micromanipulation and microscopy

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Publication details

The article was received on 03 Aug 2011, accepted on 16 Nov 2011 and first published on 14 Dec 2011


Article type: Paper
DOI: 10.1039/C1LC20719A
Citation: Lab Chip, 2012,12, 635-639
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    A multi-mode fiber probe for holographic micromanipulation and microscopy

    S. Bianchi and R. Di Leonardo, Lab Chip, 2012, 12, 635
    DOI: 10.1039/C1LC20719A

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