Logic gates for multiplexed analysis of Hg2+ and Ag+

Guiyun Zhang; Wenlie Lin; Weiqiang Yang; Zhenyu Lin; Longhua Guo; Bin Qiu; Guonan Chen

doi:10.1039/C2AN35180C

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Analyst

Issue 11, 2012

The home of premier fundamental discoveries, inventions and applications in the analytical and bioanalytical sciences

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Logic gates for multiplexed analysis of Hg²⁺ and Ag⁺

Guiyun Zhang,^a Wenlie Lin,^b Weiqiang Yang,^a Zhenyu Lin,*^a Longhua Guo,^a Bin Qiu^a and Guonan Chen^a

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MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, China
E-mail: zylin@fzu.edu.cn ;
Fax: +86-591-22866135

Yanchang Oil field Corp. Ltd., China

Analyst, 2012,137, 2687-2691

DOI: 10.1039/C2AN35180C
Received 08 Feb 2012, Accepted 13 Mar 2012
First published online 14 Mar 2012

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Abstract
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The design of devices with multiple functions, simple handling procedures and sufficient sensitivity has drawn great interests in the field of analysis. Metal–nucleotide based pairs, such as T–Hg²⁺–T and C–Ag⁺–C complexes accompanied by SYBR Green I (SG), are used to selectively bind duplex-strand DNA by observing a bright fluorescence signal in this work, thus yielding a simple method for the rapid detection of Hg²⁺ and Ag⁺ without a complex labeling process. Based on this principle, ‘OR’ and ‘AND’ logic gates for the multiplexed analysis of Hg²⁺ and Ag⁺ were developed, and their practical application for the detection of Hg²⁺ and Ag⁺ in drinking water was reported.

Abstract from Analytical Abstracts 2012

The design of devices with multiple functions, simple handling procedures and sufficient sensitivity has drawn great interests in the field of analysis. Metal-nucleotide based pairs, such as T-Hg²⁺-T and C-Ag⁺-C complexes accompanied by SYBR Green I (SG), are used to selectively bind duplex-strand DNA by observing a bright fluorescence signal in this work, thus yielding a simple method for the rapid detection of Hg²⁺ and Ag⁺ without a complex labeling process. Based on this principle, ‘OR’ and ‘AND’ logic gates for the multiplexed analysis of Hg²⁺ and Ag⁺ were developed, and their practical application for the detection of Hg²⁺ and Ag⁺ in drinking water was reported.

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Guiyun Zhang
Wenlie Lin
Weiqiang Yang
Zhenyu Lin
Longhua Guo
Bin Qiu
Guonan Chen

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