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Issue 11, 2012
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Disposable polyester–toner electrophoresis microchips for DNA analysis

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Microchip electrophoresis has become a powerful tool for DNA separation, offering all of the advantages typically associated with miniaturized techniques: high speed, high resolution, ease of automation, and great versatility for both routine and research applications. Various substrate materials have been used to produce microchips for DNA separations, including conventional (glass, silicon, and quartz) and alternative (polymers) platforms. In this study, we perform DNA separation in a simple and low-cost polyester–toner (PeT)-based electrophoresis microchip. PeT devices were fabricated by a direct-printing process using a 600 dpi-resolution laser printer. DNA separations were performed on PeT chip with channels filled with polymer solutions (0.5% m/v hydroxyethylcellulose or hydroxypropylcellulose) at electric fields ranging from 100 to 300 V cm−1. Separation of DNA fragments between 100 and 1000 bp, with good correlation of the size of DNA fragments and mobility, was achieved in this system. Although the mobility increased with increasing electric field, separations showed the same profile regardless of the electric field. The system provided good separation efficiency (215 000 plates per m for the 500 bp fragment) and the separation was completed in 4 min for 1000 bp fragment ladder. The cost of a given chip is approximately $0.15 and it takes less than 10 minutes to prepare a single device.

Graphical abstract: Disposable polyester–toner electrophoresis microchips for DNA analysis

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Publication details

The article was received on 06 Dec 2011, accepted on 24 Mar 2012 and first published on 30 Apr 2012

Article type: Paper
DOI: 10.1039/C2AN16220B
Citation: Analyst, 2012,137, 2692-2698
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    Disposable polyester–toner electrophoresis microchips for DNA analysis

    G. R. M. Duarte, W. K. T. Coltro, J. C. Borba, C. W. Price, J. P. Landers and E. Carrilho, Analyst, 2012, 137, 2692
    DOI: 10.1039/C2AN16220B

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