Using the Man9(GlcNAc)2–DC-SIGN pairing to probe specificity in photochemical immobilization

Suzanne J. Dilly; Andrew J. Clark; Daniel A. Mitchell; Andrew Marsh; Paul C. Taylor

doi:10.1039/C0MB00118J

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Using the Man₉(GlcNAc)₂–DC-SIGN pairing to probe specificity in photochemical immobilization†

Suzanne J. Dilly,^a Andrew J. Clark,^a Daniel A. Mitchell,*^b Andrew Marsh*^a and Paul C. Taylor*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
E-mail: a.marsh@warwick.ac.uk, p.c.taylor@warwick.ac.uk
Fax: +44 (0)24 7652 4112
Tel: +44 (0)24 7652 4565

^b Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, Coventry CV2 2DX, UK
E-mail: d.mitchell@warwick.ac.uk
Tel: +44 (0)24 7696 8596

Abstract

We demonstrate the expected preference of an immobilised oligosaccharide Man₉(GlcNAc)₂ upon a 96-well photochemical array, for its known receptor, the cell-surface lectin Dendritic Cell-Specific ICAM3 Grabbing Nonintegrin (DC-SIGN) when compared to immobilised competing monosaccharides.

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Supplementary files

Immobilisation of saccharides and screening versus biotin-conjugated DC-SIGN PDF (595K)

Article information

DOI: https://doi.org/10.1039/C0MB00118J
Article type: Communication
Submitted: 27 Jul 2010
Accepted: 20 Oct 2010
First published: 09 Nov 2010

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Mol. BioSyst., 2011,7, 116-118

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Using the Man₉(GlcNAc)₂–DC-SIGN pairing to probe specificity in photochemical immobilization

S. J. Dilly, A. J. Clark, D. A. Mitchell, A. Marsh and P. C. Taylor, Mol. BioSyst., 2011, 7, 116 DOI: 10.1039/C0MB00118J

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