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Issue 5, 2011
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Photoactivated capture molecule immobilization in plasmonic nanoapertures in the ultraviolet

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Abstract

We demonstrate a photoactivated surface coupling scheme for achieving spatial overlap between biomolecules of interest and optical near field excitation. Using aluminium nanoapertures, we obtained increased coupling efficiency of biotinylated capture probe oligos to the photoactivated surface due to ∼3× nanoaperture enhancement of UV light. We further validate DNA sensor functionality via the hybridization of Cy-5 labeled target oligos, with up to 8× fluorescence enhancement obtained from a commercial microarray scanner. This generic photoimmobilization strategy is an essential step to realizing miniaturized plasmon enhanced detection arrays by virtue of localizing capture molecules to the region of plasmonic enhancement.

Graphical abstract: Photoactivated capture molecule immobilization in plasmonic nanoapertures in the ultraviolet

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Publication details

The article was received on 12 Oct 2010, accepted on 10 Jan 2011 and first published on 27 Jan 2011


Article type: Communication
DOI: 10.1039/C0LC00498G
Citation: Lab Chip, 2011,11, 841-844
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    Photoactivated capture molecule immobilization in plasmonic nanoapertures in the ultraviolet

    S. Attavar, M. Diwekar and S. Blair, Lab Chip, 2011, 11, 841
    DOI: 10.1039/C0LC00498G

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