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Issue 47, 2011
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Compartmentalized reactions as a case of soft-matter biotechnology: synthesis of proteins and nucleic acids inside lipid vesicles

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Abstract

In this mini-review we would like to summarize the recent advances in the field of protein and nucleic acid synthesis inside lipid vesicles (liposomes). This research, which originated within the origin of life community, is now recognized as an example of synthetic biology. Current approaches are based on the convergence of liposome technology and cell-free in vitro technology. In particular, in addition to the classical liposome preparation methods, the new water-in-oil droplet transfer method appears very interesting for progressing in the assembly of these cell-like systems, possibly in combination with microfluidic devices. As an alternative to cell extract, the use of a transcription/translation kit composed of purified components is also presented as a new tool for carrying out protein synthesis inside liposomes. Data presented in the literature are collected and shortly discussed, and the potential relevance of this new soft-matter biotechnology in various research fields is also commented on.

Graphical abstract: Compartmentalized reactions as a case of soft-matter biotechnology: synthesis of proteins and nucleic acids inside lipid vesicles

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Publication details

The article was received on 23 May 2011, accepted on 22 Jul 2011 and first published on 04 Oct 2011


Article type: Feature Article
DOI: 10.1039/C1JM12298C
Citation: J. Mater. Chem., 2011,21, 18887-18902
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    Compartmentalized reactions as a case of soft-matter biotechnology: synthesis of proteins and nucleic acids inside lipid vesicles

    P. Stano, P. Carrara, Y. Kuruma, T. Pereira de Souza and P. L. Luisi, J. Mater. Chem., 2011, 21, 18887
    DOI: 10.1039/C1JM12298C

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