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Issue 47, 2011
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Toward low-cost biomanufacturing through in vitro synthetic biology: bottom-up design

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Abstract

While most in vitro synthetic biology projects are usually used for the purposes of basic science research or the formation of high-value products, cell-free synthetic pathway biotransformation (SyPaB), which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and coenzymes, would be used for low-cost biomanufacturing. In this article, we present bottom-up design principles for SyPaB from basic building blocks (enzymes and/or immobilized enzymes) to basic modules, such as NAD(P)H regeneration, NAD(P)H consumption, ATP regeneration, and extra ATP removal. A combination of thermostable enzymes (called thermoenzymes) with immobilization on solid supports, especially nano-materials and/or electrodes, would greatly prolong enzyme lifetime, enhance mass transfer, and facilitate product/biocatalyst separation. With developments in stable building blocks and modules (called biocatalytic modules), SyPaB has the potential to become a low-cost biomanufacturing platform for biofuels production and even biological CO2 fixation.

Graphical abstract: Toward low-cost biomanufacturing through in vitro synthetic biology: bottom-up design

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Publication details

The article was received on 10 May 2011, accepted on 10 Jun 2011 and first published on 06 Jul 2011


Article type: Feature Article
DOI: 10.1039/C1JM12078F
Citation: J. Mater. Chem., 2011,21, 18877-18886
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    Toward low-cost biomanufacturing through in vitro synthetic biology: bottom-up design

    Y.-H. P. Zhang, S. Myung, C. You, Z. Zhu and J. A. Rollin, J. Mater. Chem., 2011, 21, 18877
    DOI: 10.1039/C1JM12078F

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