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Issue 23, 2010
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Centrifugal microfluidic system for primary amplification and secondary real-time PCR

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Abstract

Pre-amplification is a basis for numerous polymerase chain reaction (PCR) protocols but bears severe contamination risks due to handling of high-copy DNA samples. Therefore we developed a self-contained centrifugal microfluidic system comprising pre-stored reagents; it enables pre-amplification of specific DNA sequences prior to automated aliquoting and real-time PCR in a modified commercial thermocycler.

Graphical abstract: Centrifugal microfluidic system for primary amplification and secondary real-time PCR

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Publication details

The article was received on 01 Jul 2010, accepted on 14 Sep 2010 and first published on 11 Oct 2010


Article type: Communication
DOI: 10.1039/C0LC00161A
Citation: Lab Chip, 2010,10, 3210-3212
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    Centrifugal microfluidic system for primary amplification and secondary real-time PCR

    M. Focke, F. Stumpf, G. Roth, R. Zengerle and F. von Stetten, Lab Chip, 2010, 10, 3210
    DOI: 10.1039/C0LC00161A

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