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Issue 23, 2010
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Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers

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Abstract

Optoelectronic tweezers was used to manipulate human spermatozoa to determine whether their response to OET predicts sperm viability among non-motile sperm. We review the electro-physical basis for how live and dead human spermatozoa respond to OET. The maximal velocity that non-motile spermatozoa could be induced to move by attraction or repulsion to a moving OET field was measured. Viable sperm are attracted to OET fields and can be induced to move at an average maximal velocity of 8.8 ± 4.2 µm s−1, while non-viable sperm are repelled to OET, and are induced to move at an average maximal velocity of −0.8 ± 1.0 µm s−1. Manipulation of the sperm using OET does not appear to result in increased DNA fragmentation, making this a potential method by which to identify viable non-motile sperm for assisted reproductive technologies.

Graphical abstract: Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers

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Publication details

The article was received on 04 Jun 2010, accepted on 12 Aug 2010 and first published on 08 Sep 2010


Article type: Paper
DOI: 10.1039/C0LC00072H
Citation: Lab Chip, 2010,10, 3213-3217
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    Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers

    A. T. Ohta, M. Garcia, J. K. Valley, L. Banie, H. Hsu, A. Jamshidi, S. L. Neale, T. Lue and M. C. Wu, Lab Chip, 2010, 10, 3213
    DOI: 10.1039/C0LC00072H

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