Jump to main content
Jump to site search

Issue 42, 2009
Previous Article Next Article

Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector

Author affiliations

Abstract

We reevaluate the absolute fluorescence and phosphorescence quantum yields of standard solutions by using a novel instrument developed for measuring the absolute emission quantum yields of solutions. The instrument consists of an integrating sphere equipped with a monochromatized Xe arc lamp as the light source and a multichannel spectrometer. By using a back-thinned CCD (BT-CCD) as the detector, the sensitivity for spectral detection in both the short and long wavelength regions is greatly improved compared with that of an optical detection system that uses a conventional photodetector. Using this instrument, we reevaluate the absolute fluorescence quantum yields (Φf) of some commonly used fluorescence standard solutions by taking into account the effect of reabsorption/reemission. The value of Φf for 5 × 10−3 M quinine bisulfate in 1 N H2SO4 is measured to be 0.52, which is in good agreement with the value (0.508) obtained by Melhuish by using a modified Vavilov method. In contrast, the value of Φf for 1.0 × 10−5 M quinine bisulfate in 1 N H2SO4, which is one of the most commonly used standards in quantum yield measurements based on the relative method, is measured to be 0.60. This value is significantly larger than Melhuish’s value (0.546), which was estimated by extrapolating the value of Φf for 5 × 10−3 M quinine bisulfate solution to infinite dilution using the self-quenching constant. The fluorescence quantum yield of 9,10-diphenylanthracene in cyclohexane is measured to be 0.97. This system can also be used to determine the phosphorescence quantum yields (Φp) of metal complexes that emit phosphorescence in the near-infrared region: the values of Φp for [Ru(bpy)3]2+ (bpy = 2,2′-bipyridine) are estimated to be 0.063 in water and 0.095 in acetonitrile under deaerated conditions at 298 K, while that in aerated water, which is frequently used as a luminescent reference in biological studies, is reevaluated to be 0.040.

Graphical abstract: Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector

Back to tab navigation

Supplementary files

Publication details

The article was received on 22 Jun 2009, accepted on 25 Aug 2009 and first published on 17 Sep 2009


Article type: Paper
DOI: 10.1039/B912178A
Citation: Phys. Chem. Chem. Phys., 2009,11, 9850-9860
  •   Request permissions

    Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector

    K. Suzuki, A. Kobayashi, S. Kaneko, K. Takehira, T. Yoshihara, H. Ishida, Y. Shiina, S. Oishi and S. Tobita, Phys. Chem. Chem. Phys., 2009, 11, 9850
    DOI: 10.1039/B912178A

Search articles by author

Spotlight

Advertisements