Toxicity testing in the 21st century: progress in the past decade and future perspectives

D. Krewski, M. E. Andersen, M. G. Tyshenko, K. Krishnan, T. Hartung, K. Boekelheide, J. F. Wambaugh, D. Jones, M. Whelan, R. Thomas, C. Yauk, T. Barton-Maclaren and I. Cote

Arch Toxicol, 2020, 94, 1
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Potential Human Health Hazard of Post-Hurricane Harvey Sediments in Galveston Bay and Houston Ship Channel: A Case Study of Using In Vitro Bioactivity Data to Inform Risk Management Decisions

Zunwei Chen, Suji Jang, James M. Kaihatu, Yi-Hui Zhou, Fred A. Wright, Weihsueh A. Chiu and Ivan Rusyn

IJERPH, 2021, 18, 13378
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Phenotypic Profiling of Reference Chemicals across Biologically Diverse Cell Types Using the Cell Painting Assay

Clinton Willis, Johanna Nyffeler and Joshua Harrill

SLAS Discovery, 2020, 25, 755
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Integrative Chemical–Biological Grouping of Complex High Production Volume Substances from Lower Olefin Manufacturing Streams

Alexandra C. Cordova, William D. Klaren, Lucie C. Ford, Fabian A. Grimm, Erin S. Baker, Yi-Hui Zhou, Fred A. Wright and Ivan Rusyn

Toxics, 2023, 11, 586
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Computational model for fetal skeletal defects potentially linked to disruption of retinoic acid signaling

Jocylin D. Pierro, Bhavesh K. Ahir, Nancy C. Baker, Nicole C. Kleinstreuer, Menghang Xia and Thomas B. Knudsen

Front. Pharmacol., 2022, 13
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In vitro cardiotoxicity assessment of environmental chemicals using an organotypic human induced pluripotent stem cell-derived model

Oksana Sirenko, Fabian A. Grimm, Kristen R. Ryan, Yasuhiro Iwata, Weihsueh A. Chiu, Frederick Parham, Jessica A. Wignall, Blake Anson, Evan F. Cromwell, Mamta Behl, Ivan Rusyn and Raymond R. Tice

Toxicology and Applied Pharmacology, 2017, 322, 60
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Development of a Zebrafish S1500+ Sentinel Gene Set for High-Throughput Transcriptomics

Michele R. Balik-Meisner, Deepak Mav, Dhiral P. Phadke, Logan J. Everett, Ruchir R. Shah, Tamara Tal, Peter J. Shepard, B. Alex Merrick and Richard S. Paules

Zebrafish, 2019, 16, 331
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Tissue-Engineered Bone Tumor as a Reproducible Human in Vitro Model for Studies of Anticancer Drugs

Courtney Sakolish, John S House, Alan Chramiec, Yizhong Liu, Zunwei Chen, Susan P Halligan, Gordana Vunjak-Novakovic and Ivan Rusyn

Toxicological Sciences, 2020, 173, 65
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Omics Advances in Ecotoxicology

Xiaowei Zhang, Pu Xia, Pingping Wang, Jianghu Yang and Donald J. Baird

Environ. Sci. Technol., 2018, 52, 3842
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Lehren aus dem Gruppieren von Chemikalien zur Bewertung der Risiken für die Gesundheit des Menschen

Wendel Wohlleben, Annette Mehling and Robert Landsiedel

Angewandte Chemie, 2023, 135
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How omics technologies can enhance chemical safety regulation: perspectives from academia, government, and industry

Bruno Campos, John K. Colbourne, James B. Brown, Mark R. Viant, Adam D. Biales, Kathryn Gallagher, Tala R. Henry, Keith G. Sappington, Stuart Marshall and Graham Whale

Environmental Toxicology and Chemistry, 2018, 37, 1252
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A Mechanistic High-Content Analysis Assay Using a Chimeric Androgen Receptor That Rapidly Characterizes Androgenic Chemicals

Adam T. Szafran, Michael J. Bolt, Caroline E. Obkirchner, Maureen G. Mancini, Christine Helsen, Frank Claessens, Fabio Stossi and Michael A. Mancini

SLAS Discovery, 2020, 25, 695
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Identifying human toxicodynamic variability: A systematic evidence map of the current knowledge

Annika Boye Petersen, Lea Bredsdorff, Natasha Tahir, Marije Niemeijer, George E.N. Kass, Aiste Vitkauskaite, Matthijs Moerland, Frederic Y. Bois, Nadia Quignot, Bob van de Water and Susanne Hougaard Bennekou

Regulatory Toxicology and Pharmacology, 2025, 161, 105842
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Cumulative Risk Meets Inter-Individual Variability: Probabilistic Concentration Addition of Complex Mixture Exposures in a Population-Based Human In Vitro Model

Suji Jang, Lucie C. Ford, Ivan Rusyn and Weihsueh A. Chiu

Toxics, 2022, 10, 549
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Repeat-dose toxicity prediction with Generalized Read-Across (GenRA) using targeted transcriptomic data: A proof-of-concept case study

Tia Tate, John Wambaugh, Grace Patlewicz and Imran Shah

Computational Toxicology, 2021, 19, 100171
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Lessons Learned from the Grouping of Chemicals to Assess Risks to Human Health

Wendel Wohlleben, Annette Mehling and Robert Landsiedel

Angew Chem Int Ed, 2023, 62
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Getting the chemicals right: Toward characterizing toxicity and ecotoxicity impacts of inorganic substances

Nienke Kirchhübel and Peter Fantke

Journal of Cleaner Production, 2019, 227, 554
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Grouping of Petroleum Substances as Example UVCBs by Ion Mobility-Mass Spectrometry to Enable Chemical Composition-Based Read-Across

Fabian A. Grimm, William K. Russell, Yu-Syuan Luo, Yasuhiro Iwata, Weihsueh A. Chiu, Tim Roy, Peter J. Boogaard, Hans B. Ketelslegers and Ivan Rusyn

Environ. Sci. Technol., 2017, 51, 7197
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ToxPi Graphical User Interface 2.0: Dynamic exploration, visualization, and sharing of integrated data models

Skylar W. Marvel, Kimberly To, Fabian A. Grimm, Fred A. Wright, Ivan Rusyn and David M. Reif

BMC Bioinformatics, 2018, 19
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Application of the 3Rs principles in the development of pharmaceutical generics

Abhijit S. Vichare, Sushant U. Kamath, Marcel Leist, A.Wallace Hayes and Brinda Mahadevan

Regulatory Toxicology and Pharmacology, 2021, 125, 105016
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Dosing Methods to Enable Cell-Based In Vitro Testing of Complex Substances: A Case Study with a PAH Mixture

Alexandra C. Cordova, Lucie C. Ford, Alan Valdiviezo, Alina T. Roman-Hubers, Thomas J. McDonald, Weihsueh A. Chiu and Ivan Rusyn

Toxics, 2022, 11, 19
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Utility of in vitro assay data in read-across prediction of nongenotoxic carcinogenicity of pesticides for cytotoxicity-related rat tumors

Kosuke Mizuno, Jun-ichi Takeshita, Yu Harakawa, Takuomi Hosaka, Ryota Shizu and Kouichi Yoshinari

Food and Chemical Toxicology, 2025, 202, 115566
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Chemical and biological assessments of environmental mixtures: A review of current trends, advances, and future perspectives

Yu-Syuan Luo, Zunwei Chen, Nan-Hung Hsieh and Tzu-En Lin

Journal of Hazardous Materials, 2022, 432, 128658
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Comparison of Gene Expression Responses in the Small Intestine of Mice Following Exposure to 3 Carcinogens Using the S1500+ Gene Set Informs a Potential Common Adverse Outcome Pathway

Grace A. Chappell, Julia E. Rager, Jeffrey Wolf, Milos Babic, Kyle J. LeBlanc, Caroline L. Ring, Mark A. Harris and Chad M. Thompson

Toxicol Pathol, 2019, 47, 851
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Qualitative Approach to Comparative Exposure in Alternatives Assessment

William Greggs, Thomas Burns, Peter Egeghy, Michelle R Embry, Peter Fantke, Bonnie Gaborek, Lauren Heine, Olivier Jolliet, Carolyn Lee, Derek Muir, Kathy Plotzke, Joseph Rinkevich, Neha Sunger, Jennifer Y Tanir and Margaret Whittaker

Integrated Environmental Assessment and Management, 2019, 15, 880
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Enzymatic Hydrolysis in the Green Production of Bacterial Cellulose Nanocrystals

Cesare Rovera, Masoud Ghaani, Nadia Santo, Silvia Trabattoni, Richard T. Olsson, Diego Romano and Stefano Farris

ACS Sustainable Chem. Eng., 2018, 6, 7725
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Metabolomics as read-across tool: An example with 3-aminopropanol and 2-aminoethanol

S. Sperber, M. Wahl, F. Berger, H. Kamp, O. Lemke, V. Starck, T. Walk, M. Spitzer and B.v Ravenzwaay

Regulatory Toxicology and Pharmacology, 2019, 108, 104442
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Analytical chemistry solutions to hazard evaluation of petroleum refining products

Alina T. Roman-Hubers, Alexandra C. Cordova, Mark P. Barrow and Ivan Rusyn

Regulatory Toxicology and Pharmacology, 2023, 137, 105310
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Extraction-free whole transcriptome gene expression analysis of FFPE sections and histology-directed subareas of tissue

Christy L. Trejo, Miloš Babić, Elliot Imler, Migdalia Gonzalez, Sergei I. Bibikov, Peter J. Shepard, Harper C. VanSteenhouse, Joanne M. Yeakley, Bruce E. Seligmann and Francisco X. Real

PLoS ONE, 2019, 14, e0212031
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In Vitro Bioavailability of the Hydrocarbon Fractions of Dimethyl Sulfoxide Extracts of Petroleum Substances

Yu-Syuan Luo, Kyle C Ferguson, Ivan Rusyn and Weihsueh A Chiu

Toxicological Sciences, 2020, 174, 168
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A Pipeline for High-Throughput Concentration Response Modeling of Gene Expression for Toxicogenomics

John S. House, Fabian A. Grimm, Dereje D. Jima, Yi-Hui Zhou, Ivan Rusyn and Fred A. Wright

Front. Genet., 2017, 8
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Multi-dimensional in vitro bioactivity profiling for grouping of glycol ethers

Fabian A. Grimm, John S. House, Melinda R. Wilson, Oksana Sirenko, Yasuhiro Iwata, Fred A. Wright, Nicholas Ball and Ivan Rusyn

Regulatory Toxicology and Pharmacology, 2019, 101, 91
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Relationships between constituents of energy drinks and beating parameters in human induced pluripotent stem cell (iPSC)-Derived cardiomyocytes

Yu-Syuan Luo, Zunwei Chen, Alexander D. Blanchette, Yi-Hui Zhou, Fred A. Wright, Erin S. Baker, Weihsueh A. Chiu and Ivan Rusyn

Food and Chemical Toxicology, 2021, 149, 111979
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Regulatory acceptance of in silico approaches for the safety assessment of cosmetic-related substances

Nichola Gellatly and Fiona Sewell

Computational Toxicology, 2019, 11, 82
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Natasha R. Catlin, Bradley J. Collins, Scott S. Auerbach, Stephen S. Ferguson, James M. Harnly, Chris Gennings, Suramya Waidyanatha, Glenn E. Rice, Stephanie L. Smith-Roe, Kristine L. Witt and Cynthia V. Rider

Food and Chemical Toxicology, 2018, 118, 328
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Transcriptomic pathway and benchmark dose analysis of Bisphenol A, Bisphenol S, Bisphenol F, and 3,3',5,5'-Tetrabromobisphenol A in H9 human embryonic stem cells

Vian Peshdary, Cheryl A. Hobbs, Timothy Maynor, Kim Shepard, Remi Gagné, Andrew Williams, Byron Kuo, Nikolai Chepelev, Leslie Recio, Carole Yauk and Ella Atlas

Toxicology in Vitro, 2021, 72, 105097
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Phenotypic Assays for Characterizing Compound Effects on Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

Oksana Sirenko, Michael K. Hancock, Carole Crittenden, Matthew Hammer, Sean Keating, Coby B. Carlson and Grischa Chandy

ASSAY and Drug Development Technologies, 2017, 15, 280
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Technology Transfer of the Microphysiological Systems: A Case Study of the Human Proximal Tubule Tissue Chip

Courtney Sakolish, Elijah J. Weber, Edward J. Kelly, Jonathan Himmelfarb, Roula Mouneimne, Fabian A. Grimm, John S. House, Terry Wade, Arum Han, Weihsueh A. Chiu and Ivan Rusyn

Sci Rep, 2018, 8
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The Impact of Novel Assessment Methodologies in Toxicology on Green Chemistry and Chemical Alternatives

Ivan Rusyn and Nigel Greene

Toxicological Sciences, 2018, 161, 276
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A Tox21 Approach to Altered Epigenetic Landscapes: Assessing Epigenetic Toxicity Pathways Leading to Altered Gene Expression and Oncogenic Transformation In Vitro

Craig Parfett and Daniel Desaulniers

IJMS, 2017, 18, 1179
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Hexabromocyclododecane (HBCD): A case study applying tiered testing for human health risk assessment

Anne Marie Gannon, Marjory Moreau, Reza Farmahin, Russell S. Thomas, Tara S. Barton-Maclaren, Andy Nong, Ivan Curran and Carole L. Yauk

Food and Chemical Toxicology, 2019, 131, 110581
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Combined Exposures and Mixtures Research: An Enduring NIEHS Priority

Danielle J. Carlin and Cynthia V. Rider

Environ Health Perspect, 2024, 132
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Whole-transcriptome sequencing identified gene expression signatures associated with aggressive clear cell renal cell carcinoma

Ken Batai, Elliot Imler, Jayce Pangilinan, Robert Bell, Aye Lwin, Elinora Price, Tijana Milinic, Amit Arora, Nathan A. Ellis, Erika Bracamonte, Bruce Seligmann and Benjamin R. Lee

Genes Cancer, 2018, 9, 247
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A cross-sector call to improve carcinogenicity risk assessment through use of genomic methodologies

Carole L. Yauk, Alison H. Harrill, Heidrun Ellinger-Ziegelbauer, Jan Willem van der Laan, Jonathan Moggs, Roland Froetschl, Frank Sistare and Syril Pettit

Regulatory Toxicology and Pharmacology, 2020, 110, 104526
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A tiered testing strategy based on in vitro phenotypic and transcriptomic data for selecting representative petroleum UVCBs for toxicity evaluation in vivo

Han-Hsuan Doris Tsai, John S House, Fred A Wright, Weihsueh A Chiu and Ivan Rusyn

Toxicological Sciences, 2023, 193, 219
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Integration of Epigenetic Mechanisms into Non-Genotoxic Carcinogenicity Hazard Assessment: Focus on DNA Methylation and Histone Modifications

Daniel Desaulniers, Paule Vasseur, Abigail Jacobs, M. Cecilia Aguila, Norman Ertych and Miriam N. Jacobs

IJMS, 2021, 22, 10969
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A Comparison of the TempO-Seq S1500+ Platform to RNA-Seq and Microarray Using Rat Liver Mode of Action Samples

Pierre R. Bushel, Richard S. Paules and Scott S. Auerbach

Front. Genet., 2018, 9
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Pathway-based predictive approaches for non-animal assessment of acute inhalation toxicity

Amy J. Clippinger, David Allen, Holger Behrsing, Kelly A. BéruBé, Michael B. Bolger, Warren Casey, Michael DeLorme, Marianna Gaça, Sean C. Gehen, Kyle Glover, Patrick Hayden, Paul Hinderliter, Jon A. Hotchkiss, Anita Iskandar, Brian Keyser, Karsta Luettich, Lan Ma-Hock, Anna G. Maione, Patrudu Makena, Jodie Melbourne, Lawrence Milchak, Sheung P. Ng, Alicia Paini, Kathryn Page, Grace Patlewicz, Pilar Prieto, Hans Raabe, Emily N. Reinke, Clive Roper, Jane Rose, Monita Sharma, Wayne Spoo, Peter S. Thorne, Daniel M. Wilson and Annie M. Jarabek

Toxicology in Vitro, 2018, 52, 131
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Utility of Extrapolating Human S1500+ Genes to the Whole Transcriptome: Tunicamycin Case Study

Deepak Mav, Dhiral P Phadke, Michele R Balik-Meisner, B Alex Merrick, Scott Auerbach, Marije Niemeijer, Suzanna Huppelschoten, Audrey Baze, Celine Parmentier, Lysiane Richert, Bob van de Water, Ruchir R Shah and Richard S Paules

Bioinform Biol Insights, 2020, 14, 117793222095274
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Alternatives assessment: An analysis on progress and future needs for research and practice

Aude M. Bechu, Monika A. Roy, Molly Jacobs and Joel A. Tickner

Integrated Environmental Assessment and Management, 2024, 20, 1337
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Risk Characterization of Environmental Samples UsingIn VitroBioactivity and Polycyclic Aromatic Hydrocarbon Concentrations Data

Zunwei Chen, Dillon Lloyd, Yi-Hui Zhou, Weihsueh A Chiu, Fred A Wright and Ivan Rusyn

Toxicological Sciences, 2021, 179, 108
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Toward Rigorous Materials Production: New Approach Methodologies Have Extensive Potential to Improve Current Safety Assessment Practices

Penny Nymark, Martine Bakker, Susan Dekkers, Remy Franken, Wouter Fransman, Amaia García‐Bilbao, Dario Greco, Mary Gulumian, Niels Hadrup, Sabina Halappanavar, Vesa Hongisto, Karin Sørig Hougaard, Keld Alstrup Jensen, Pekka Kohonen, Antti Joonas Koivisto, Miikka Dal Maso, Thies Oosterwijk, Mikko Poikkimäki, Isabel Rodriguez‐Llopis, Rob Stierum, Jorid Birkelund Sørli and Roland Grafström

Small, 2020, 16
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Comparison of Normalization Methods for Analysis of TempO-Seq Targeted RNA Sequencing Data

Pierre R. Bushel, Stephen S. Ferguson, Sreenivasa C. Ramaiahgari, Richard S. Paules and Scott S. Auerbach

Front. Genet., 2020, 11
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Analysis of reproducibility and robustness of a renal proximal tubule microphysiological system OrganoPlate 3-lane 40 for in vitro studies of drug transport and toxicity

Courtney Sakolish, Haley L Moyer, Han-Hsuan D Tsai, Lucie C Ford, Allison N Dickey, Fred A Wright, Gang Han, Piyush Bajaj, Maria T Baltazar, Paul L Carmichael, Jason P Stanko, Stephen S Ferguson and Ivan Rusyn

Toxicological Sciences, 2023, 196, 52
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Toxicity bioassay of waste cooking oil-based biodiesel on marine microalgae

K.S. Pikula, A.M. Zakharenko, V.V. Chaika, A.K. Stratidakis, M. Kokkinakis, G. Waissi, V.N. Rakitskii, D.A. Sarigiannis, A.W. Hayes, M.D. Coleman, A. Tsatsakis and K.S. Golokhvast

Toxicology Reports, 2019, 6, 111
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Comparison of base-line and chemical-induced transcriptomic responses in HepaRG and RPTEC/TERT1 cells using TempO-Seq

Alice Limonciel, Gamze Ates, Giada Carta, Anja Wilmes, Manfred Watzele, Peter J. Shepard, Harper C. VanSteenhouse, Bruce Seligmann, Joanne M. Yeakley, Bob van de Water, Mathieu Vinken and Paul Jennings

Arch Toxicol, 2018, 92, 2517
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