Issue 16, 2024

Capture-and-release of a sulfoquinovose-binding protein on sulfoquinovose-modified agarose

Abstract

The solute-binding protein (SBP) components of periplasmic binding protein-dependent ATP-binding cassette (ABC)-type transporters often possess exquisite selectivity for their cognate ligands. Maltose binding protein (MBP), the best studied of these SBPs, has been extensively used as a fusion partner to enable the affinity purification of recombinant proteins. However, other SBPs and SBP-ligand based affinity systems remain underexplored. The sulfoquinovose-binding protein SmoF, is a substrate-binding protein component of the ABC transporter cassette in Agrobacterium tumefaciens involved in importing sulfoquinovose (SQ) and its derivatives for SQ catabolism. Here, we show that SmoF binds with high affinity to the octyl glycoside of SQ (octyl-SQ), demonstrating remarkable tolerance to extension of the anomeric substituent. The 3D X-ray structure of the SmoF·octyl-SQ complex reveals accommodation of the octyl chain, which projects to the protein surface, providing impetus for the synthesis of a linker-equipped SQ-amine using a thiol–ene reaction as a key step, and its conjugation to cyanogen bromide modified agarose. We demonstrate the successful capture and release of SmoF from SQ-agarose resin using SQ as competitive eluant, and selectivity for release versus other organosulfonates. We show that SmoF can be captured and purified from a cell lysate, demonstrating the utility of SQ-agarose in capturing SQ binding proteins from complex mixtures. The present work provides a pathway for development of ‘capture-and-release’ affinity resins for the discovery and study of SBPs.

Graphical abstract: Capture-and-release of a sulfoquinovose-binding protein on sulfoquinovose-modified agarose

Supplementary files

Article information

Article type
Paper
Submitted
27 Feb 2024
Accepted
24 Mas 2024
First published
25 Mas 2024

Org. Biomol. Chem., 2024,22, 3237-3244

Capture-and-release of a sulfoquinovose-binding protein on sulfoquinovose-modified agarose

T. Arumapperuma, A. J. D. Snow, M. Lee, M. Sharma, Y. Zhang, J. P. Lingford, E. D. Goddard-Borger, G. J. Davies and S. J. Williams, Org. Biomol. Chem., 2024, 22, 3237 DOI: 10.1039/D4OB00307A

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