Issue 9, 2024

Advancements in molecular disassembly of optical probes: a paradigm shift in sensing, bioimaging, and therapeutics

Abstract

The majority of self-assembled fluorescent dyes suffer from aggregation-caused quenching (ACQ), which detrimentally affects their diagnostic and therapeutic effectiveness. While aggregation-induced emission (AIE) active dyes offer a promising solution to overcome this limitation, they may face significant challenges as the intracellular environment often prevents aggregation, leading to disassembly and posing challenges for AIE fluorogens. Recent progress in signal amplification through the disassembly of ACQ dyes has opened new avenues for creating ultrasensitive optical sensors and enhancing phototherapeutic outcomes. These advances are well-aligned with cutting-edge technologies such as single-molecule microscopy and targeted molecular therapies. This work explores the concept of disaggregation-induced emission (DIE), showcasing the revolutionary capabilities of DIE-based dyes from their design to their application in sensing, bioimaging, disease monitoring, and treatment in both cellular and animal models. Our objective is to provide an in-depth comparison of aggregation versus disaggregation mechanisms, aiming to stimulate further advancements in the design and utilization of ACQ fluorescent dyes through DIE technology. This initiative is poised to catalyze scientific progress across a broad spectrum of disciplines.

Graphical abstract: Advancements in molecular disassembly of optical probes: a paradigm shift in sensing, bioimaging, and therapeutics

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Article information

Article type
Review Article
Submitted
30 Eph 2024
Accepted
18 Jun 2024
First published
04 Jul 2024
This article is Open Access
Creative Commons BY license

Nanoscale Horiz., 2024,9, 1390-1416

Advancements in molecular disassembly of optical probes: a paradigm shift in sensing, bioimaging, and therapeutics

K. Saczuk, M. Dudek, K. Matczyszyn and M. Deiana, Nanoscale Horiz., 2024, 9, 1390 DOI: 10.1039/D4NH00186A

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