Issue 16, 2022

All-printed point-of-care immunosensing biochip for one drop blood diagnostics

Abstract

Designing and preparing a fast and easy-to-use immunosensing biochip are of great significance for clinical diagnosis and biomedical research. In particular, sensitive, specific, and early detection of biomarkers in trace samples promotes the application of point-of-care testing (POCT). Here, we demonstrate an all-printed immunosensing biochip with the characteristics of hydrodynamic enrichment and photonic crystal-enhanced fluorescence. Direct quantitative detection of cardiac biomarkers via one drop of blood is achieved in 10 min. After simulating the hydrodynamic behavior of one droplet serum on the printed assay, creatine kinase-MB (CK-MB) has been recognized and located on the photonic crystal arrays. Benefiting from the fluorescence enhancement effect, quantitative detection of CK-MB has been demonstrated from 0.01 ng ml−1 to 100 ng ml−1, which is superior to the conventional enzyme-linked immunosorbent assay (ELISA). This strategy provides a general and easy-to-use approach for fast quantitative detection of biomarkers, which would be improved further for portable clinical diagnostics and home medical monitoring.

Graphical abstract: All-printed point-of-care immunosensing biochip for one drop blood diagnostics

Supplementary files

Article information

Article type
Paper
Submitted
26 Eph 2022
Accepted
17 Jun 2022
First published
21 Jun 2022

Lab Chip, 2022,22, 3008-3014

All-printed point-of-care immunosensing biochip for one drop blood diagnostics

J. Chi, Y. Wu, F. Qin, M. Su, N. Cheng, J. Zhang, C. Li, Z. Lian, X. Yang, L. Cheng, H. Xie, H. Wang, Z. Zhang, J. Carmeliet and Y. Song, Lab Chip, 2022, 22, 3008 DOI: 10.1039/D2LC00385F

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