Issue 58, 2016
From the journal:

Chemical Communications

Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance†

Lindsey A. Flanagan,a   John J. Wright,b   Maxie M. Roessler,b   James W. Moirc  and  Alison Parkin*a  

* Corresponding authors

a Department of Chemistry, University of York, Heslington, York, UK
E-mail: alison.parkin@york.ac.uk
Tel: +44 1904 322561

b School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK

c Department of Biology, University of York, York YO10 5DD, UK

Abstract

Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.

Graphical abstract: Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C6CC00515B
Article type
Communication
Submitted
18 Jan 2016
Accepted
31 Mas 2016
First published
31 Mas 2016
This article is Open Access
Creative Commons BY license

Chem. Commun., 2016,52, 9133-9136

Author version available

Download author version (PDF)

Permissions

Request permissions

Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

L. A. Flanagan, J. J. Wright, M. M. Roessler, J. W. Moir and A. Parkin, Chem. Commun., 2016, 52, 9133 DOI: 10.1039/C6CC00515B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements