A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry

Amandeep Kaur; Mohammad A. Haghighatbin; Conor F. Hogan; Elizabeth J. New

doi:10.1039/C5CC03394B

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A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry†

Amandeep Kaur,^a Mohammad A. Haghighatbin,^b Conor F. Hogan^b and Elizabeth J. New*^a

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* Corresponding authors

^a School of Chemistry, The University of Sydney, New South Wales, Australia
E-mail: elizabeth.new@sydney.edu.au
Fax: +612 9351 3329
Tel: +612 9351 1993

^b Department of Chemistry, La Trobe Institute for Molecular Sciences, La Trobe University, Melbourne, Victoria 3086, Australia

Abstract

Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities – confocal microscopy, fluorescence lifetime imaging and flow cytometry.

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Article information

DOI: https://doi.org/10.1039/C5CC03394B
Article type: Communication
Submitted: 23 Eph 2015
Accepted: 29 Mey 2015
First published: 03 Jun 2015

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Chem. Commun., 2015,51, 10510-10513

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A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry

A. Kaur, M. A. Haghighatbin, C. F. Hogan and E. J. New, Chem. Commun., 2015, 51, 10510 DOI: 10.1039/C5CC03394B

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Chemical Communications

A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry†

Abstract

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A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry

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