Issue 8, 2018

Superhydrophobic polypyrene films to prevent Staphylococcus aureus and Pseudomonas aeruginosa biofilm adhesion on surfaces: high efficiency deciphered by fluorescence microscopy

Abstract

A blue luminescent and superhydrophobic coating based on an electropolymerized fluorinated-pyrene monomer and its planktonic bacteria and biofilm repellent properties are reported. Two different pathogenic bacterial strains (Gram-positive and Gram-negative) at two different incubation times (2 h planktonic bacterial and 24 h biofilm adhesion) were studied and monitored (analyzed) using multicolor scanning confocal fluorescence microscopy. The coating was proved to reduce bacterial adhesion by 65%. It is highly effective against biofilm attachment, with 90% reduction of bacteria surface coverage. This blue fluorescent surface provides a facile method to characterize the coating, observe the bacterial distribution and quantify the bacterial coverage rate by fluorescence imaging of different colors. Furthermore, the film does not show significant bacterial toxicity during the working incubation times.

Graphical abstract: Superhydrophobic polypyrene films to prevent Staphylococcus aureus and Pseudomonas aeruginosa biofilm adhesion on surfaces: high efficiency deciphered by fluorescence microscopy

Supplementary files

Article information

Article type
Paper
Submitted
29 1月 2018
Accepted
09 5月 2018
First published
10 5月 2018

Photochem. Photobiol. Sci., 2018,17, 1023-1035

Superhydrophobic polypyrene films to prevent Staphylococcus aureus and Pseudomonas aeruginosa biofilm adhesion on surfaces: high efficiency deciphered by fluorescence microscopy

G. Morán, G. Ramos-Chagas, S. Hugelier, X. Xie, R. Boudjemaa, C. Ruckebusch, M. Sliwa, T. Darmanin, A. Gaucher, D. Prim, G. Godeau, S. Amigoni, F. Guittard and R. Méallet-Renault, Photochem. Photobiol. Sci., 2018, 17, 1023 DOI: 10.1039/C8PP00043C

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