Issue 30, 2022

Rapid and sensitive naked eye detection of faecal pigments using their enhanced solid-state green fluorescence on a zinc acetate substrate

Abstract

The identification of trace faecal pigments in real-time and on-site detection remains a challenge for water quality monitoring. Herein, a simple, low-cost and rapid fluorescence-based analytical method has been developed in a solid matrix for faecal pigments like stercobilin and urobilin detection. This was made possible due to significant enhancement of green solid-state fluorescence (520 nm) by zinc(II) complexation with faecal pigments embedded in the surface of zinc acetate crystals. It enables naked-eye detection of these pigments even at a 10 μM level when excited with 365 nm blue-UV. It was demonstrated that easily available white cellulose paper strips or TLC silica plates coated with zinc acetate can be used as substrates. A photophysical study of solid-state faecal pigments–zinc(II) complexes suggests that green fluorescence enhancement results from the complexation, which can be attributed to the substantial decrease of the non-radiative decay rate (knr) as well as more efficient use of excitation light. The observation of reduced interference of humic acid fluorescence makes faecal pigment detection more efficient by this proposed method.

Graphical abstract: Rapid and sensitive naked eye detection of faecal pigments using their enhanced solid-state green fluorescence on a zinc acetate substrate

Supplementary files

Article information

Article type
Communication
Submitted
30 5月 2022
Accepted
06 7月 2022
First published
07 7月 2022

Anal. Methods, 2022,14, 2907-2912

Rapid and sensitive naked eye detection of faecal pigments using their enhanced solid-state green fluorescence on a zinc acetate substrate

S. Prakash and A. K. Mishra, Anal. Methods, 2022, 14, 2907 DOI: 10.1039/D2AY00878E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements