Issue 3, 2021

An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells

Abstract

Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vitro enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform.

Graphical abstract: An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells

Supplementary files

Article information

Article type
Communication
Submitted
11 12月 2020
Accepted
04 3月 2021
First published
15 3月 2021
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2021,2, 830-834

An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells

R. A. Baglia, K. R. Mills, K. Mitra, J. N. Tutol, D. Ball, K. M. Page, J. Kallu, S. Gottipolu, S. D’Arcy, S. O. Nielsen and S. C. Dodani, RSC Chem. Biol., 2021, 2, 830 DOI: 10.1039/D0CB00231C

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