Issue 3, 2020

Developmental toxicity of trichloroethylene in zebrafish (Danio rerio)

Abstract

Trichloroethylene (TCE), an industrial solvent and degreaser, is an environmental toxicant that contaminates over half of Superfund sites, is a known carcinogen, and is linked to congenital defects and neurodegenerative disease. The developmental toxicity of TCE near ecologically relevant levels needs further characterization in order to better assess health risks of exposure. In this study, the toxicodynamics of TCE in the zebrafish (Danio rerio) model was investigated through the establishment of a LC50 concentration and by monitoring the acute developmental toxicity of ecologically relevant concentrations (0, 5, 50, and 500 parts per billion; ppb) of TCE during two different exposure lengths (1–72 hours post fertilization (hpf) and 1–120 hpf). Acute developmental toxicity was assessed by monitoring survival and hatching, larval morphology, larval heart rate, and behavioral responses during an embryonic photomotor response test and a larval visual motor response test. Embryonic exposure to TCE was associated with decreased percent hatch at 48 hpf, altered larval morphology, increased heart rate, and altered behavioral responses during the photomotor response test and visual motor response test. Larval morphology and behavioral alterations were more pronounced in the 1–120 hpf exposure length trials. The observed alterations suggest developmental TCE toxicity is still a concern at regulatory concentrations and that timing of exposure influences developmental toxicity.

Graphical abstract: Developmental toxicity of trichloroethylene in zebrafish (Danio rerio)

Supplementary files

Article information

Article type
Paper
Submitted
03 12月 2019
Accepted
22 1月 2020
First published
23 1月 2020

Environ. Sci.: Processes Impacts, 2020,22, 728-739

Developmental toxicity of trichloroethylene in zebrafish (Danio rerio)

K. A. Horzmann, A. M. Portales, K. G. Batcho and J. L. Freeman, Environ. Sci.: Processes Impacts, 2020, 22, 728 DOI: 10.1039/C9EM00565J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements