Issue 3, 2019

Gold nanoparticle coatings as efficient adenovirus carriers to non-infectable stem cells

Abstract

Mesenchymal stem cells (MSCs) are adult pluripotent cells with the plasticity to be converted into different cell types. Their self-renewal capacity, relative ease of isolation, expansion and inherent migration to tumors, make them perfect candidates for cell therapy against cancer. However, MSCs are notoriously refractory to adenoviral infection, mainly because CAR (Coxsackie-Adenovirus Receptor) expression is absent or downregulated. Over the last years, nanoparticles have attracted a great deal of attention as potential vehicle candidates for gene delivery, but with limited effects on their own. Our data showed that the use of positively charged 14 nm gold nanoparticles either functionalized with arginine–glycine–aspartate (RGD) motif or not, increases the efficiency of adenovirus infection in comparison to commercial reagents without altering cell viability or cell phenotype. This system represents a simple, efficient and safe method for the transduction of MSCs, being attractive for cancer gene and cell therapies.

Graphical abstract: Gold nanoparticle coatings as efficient adenovirus carriers to non-infectable stem cells

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
02 11月 2018
Accepted
24 12月 2018
First published
10 1月 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 1327-1334

Gold nanoparticle coatings as efficient adenovirus carriers to non-infectable stem cells

Y. Hernandez, R. González-Pastor, C. Belmar-Lopez, G. Mendoza, J. M. de la Fuente and P. Martin-Duque, RSC Adv., 2019, 9, 1327 DOI: 10.1039/C8RA09088B

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements