Issue 7, 2019

Extracellular vesicles as cancer liquid biopsies: from discovery, validation, to clinical application

Abstract

Substantial research has been devoted to elucidate the roles that extracellular vesicles (EVs) play in the regulation of both normal and pathological processes, and multiple studies have demonstrated their potential as a source of cancer biomarkers. However, several factors have slowed the development of liquid biopsy EV biomarkers for cancer diagnosis, including logistical and technical difficulties associated with reproducibly obtaining highly purified EVs suitable for diagnostic analysis. Significant effort has focused on addressing these problems, and multiple groups have now reported EV analysis methods using liquid biopsies that have the potential for clinical translation. However, there are still important issues that must be addressed if these discoveries and technical advances are to be used for clinical translation of EV cancer biomarkers from liquid biopsies. To address these issues, this review focuses on the potential application of EV biomarkers for diagnosis of major cancer types, discussing approaches for EV biomarker discovery and verification, EV clinical assay development, analytical and clinical validation, clinical trials, regulatory submission, and end user utilization for the intended clinical application. This review also discusses key difficulties related to these steps, and recommendations for how to best accomplish steps in order to translate EV-based biomarkers into clinical settings.

Graphical abstract: Extracellular vesicles as cancer liquid biopsies: from discovery, validation, to clinical application

Article information

Article type
Tutorial Review
Submitted
20 10月 2018
Accepted
04 3月 2019
First published
05 3月 2019

Lab Chip, 2019,19, 1114-1140

Extracellular vesicles as cancer liquid biopsies: from discovery, validation, to clinical application

Z. Zhao, J. Fan, Y. S. Hsu, C. J. Lyon, B. Ning and T. Y. Hu, Lab Chip, 2019, 19, 1114 DOI: 10.1039/C8LC01123K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements