Issue 2, 2016

Rapid bladder cancer cell detection from clinical urine samples using an ultra-thin silicone membrane

Abstract

Early detection of initial onset, as well as recurrence, of cancer is paramount for improved patient prognosis and human health. Cancer screening is enhanced by rapid differentiation of cancerous from non-cancerous cells which employs the inherent differences in biophysical properties. Our preliminary testing demonstrates that cell-line derived bladder cancer cells deform our <30 nm silicone membrane within an hour and induce visually distinct wrinkle patterns while cell-line derived non-cancerous cells fail to induce these wrinkle patterns. Herein, we report a platform for the rapid detection of cancerous cells from human clinical urine samples. We performed a blinded study with cells extracted from the urine of human patients suspected to have bladder cancer alongside healthy controls. Wrinkle patterns were induced specifically by the five cancer patient samples within 12 hours and not by the healthy controls. These results were independently validated by the standard diagnostic techniques cystoscopy and cytology. Thus, our ultra-thin membrane approach for cancer diagnosis appears as accurate as standard diagnostic methods while vastly more rapid, less invasive, and requiring limited expertise.

Graphical abstract: Rapid bladder cancer cell detection from clinical urine samples using an ultra-thin silicone membrane

Supplementary files

Article information

Article type
Paper
Submitted
07 8月 2015
Accepted
30 10月 2015
First published
09 11月 2015

Analyst, 2016,141, 652-660

Rapid bladder cancer cell detection from clinical urine samples using an ultra-thin silicone membrane

J. H. Appel, H. Ren, M. L. Y. Sin, J. C. Liao and J. Chae, Analyst, 2016, 141, 652 DOI: 10.1039/C5AN01616A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements