Issue 17, 2015

Colorimetric determination of nitrite in clinical, food and environmental samples using microfluidic devices stamped in paper platforms

Abstract

This study reports the use of microfluidic paper-based analytical devices (μPADs) associated with colorimetric detection for the determination of nitrite in clinical, food and environmental samples. μPADs were fabricated by a simple and fast stamping process in a geometry containing eight circular detection zones and one central zone to sample inlet interconnected by microfluidic channels. The colorimetric determination of nitrite was performed through the modified Griess reaction. Detection zones were spotted with a 0.75 μL aliquot of a solution containing 50 mM sulfanilamide, 1.2 M hydrochloric acid and 4 mM N-(1-naphthyl)ethylenediamine. The monitoring of the background colorimetric response revealed good stability over 12 h for devices stored in the absence of light. After the addition of standard or real samples, the resulting images were captured with a scanner, converted to a color scale and analyzed in the magenta channel. The analytical sensitivity and the limit of detection achieved after a preconcentration stage were 0.56 (AU μM−1) and 5.6 μM, respectively. The preconcentration provided an enrichment factor of ca. 3.2 times. The concentration levels of nitrite were successfully determined in saliva, preservative water, ham, sausage and river water samples. The concentration levels attained for each sample using μPADs were compared to the values found by spectrophotometry and there was no significant difference from one another at a confidence level of 95%.

Graphical abstract: Colorimetric determination of nitrite in clinical, food and environmental samples using microfluidic devices stamped in paper platforms

Article information

Article type
Paper
Submitted
20 2月 2015
Accepted
30 4月 2015
First published
06 5月 2015

Anal. Methods, 2015,7, 7311-7317

Colorimetric determination of nitrite in clinical, food and environmental samples using microfluidic devices stamped in paper platforms

T. M. G. Cardoso, P. T. Garcia and W. K. T. Coltro, Anal. Methods, 2015, 7, 7311 DOI: 10.1039/C5AY00466G

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