Toward a unified pipeline for natural product discovery: tools and strategies for NRPS and PKS pathway exploration and engineering

Biyan Chen, Emre F. Bülbül, SeoungGun Bang, Hannah A. Minas and Kenan A. J. Bozhüyük

Nat. Prod. Rep., 2025
DOI: 10.1039/D5NP00041F

Peptidic Tryptophan Halogenation by a Promiscuous Flavin‐Dependent Enzyme

Andrew J. Rice, Mayuresh G. Gadgil, Paola Bisignano, Richard A. Stein, Hassane S. Mchaourab and Douglas A. Mitchell

Angewandte Chemie, 2025, 137
DOI: 10.1002/ange.202509729

Advances in the microbial biosynthesis of therapeutic terpenoids

Peter H Winegar, Maria CT Astolfi, Sara F Holm, Graham A Hudson and Jay D Keasling

Current Opinion in Biotechnology, 2025, 96, 103374
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Characterizing and engineering post-translational modifications with high-throughput cell-free expression

Derek A. Wong, Zachary M. Shaver, Maria D. Cabezas, Martin Daniel-Ivad, Katherine F. Warfel, Deepali V. Prasanna, Sarah E. Sobol, Regina Fernandez, Fernando Tobias, Szymon K. Filip, Sophia W. Hulbert, Peter Faull, Robert Nicol, Matthew P. DeLisa, Emily P. Balskus, Ashty S. Karim and Michael C. Jewett

Nat Commun, 2025, 16
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Amino acid tailoring strategies in peptide natural product biosynthesis

Lukas Zimmer and Max Crüsemann

Trends in Chemistry, 2025
DOI: 10.1016/j.trechm.2025.09.014

Peptidic Tryptophan Halogenation by a Promiscuous Flavin‐Dependent Enzyme

Andrew J. Rice, Mayuresh G. Gadgil, Paola Bisignano, Richard A. Stein, Hassane S. Mchaourab and Douglas A. Mitchell

Angew Chem Int Ed, 2025, 64
DOI: 10.1002/anie.202509729

Cell-Free Protein Synthesis Reactor Formats: A Brief History and Analysis

Dallin M. Chipman, Anna C. Woolley, Davu N. Chau, William A. Lance, Joseph P. Talley, Tyler P. Green, Benjamin C. Robbins and Bradley C. Bundy

SynBio, 2025, 3, 10
DOI: 10.3390/synbio3030010

Endophytic fungi as biocontrol agents: A metabolite-driven approach to crop protection and sustainable agriculture

Muhammad Faiq, Amjad Ali, Shazia Shafique, Sobiya Shafique, Allah Rakha Yaseen, Rimsha Fatima, Muhammad Tanveer Altaf and Faheem Shehzad Baloch

Physiological and Molecular Plant Pathology, 2025, 140, 102857
DOI: 10.1016/j.pmpp.2025.102857

Protein Engineering of Biosynthetic Enzymes Unlocks Libraries of Bioactive Tilimycin Analogs

Monica R. MacDonald, James Hasselbeck and Andrew M. Gulick

ACS Catal., 2025, 17801
DOI: 10.1021/acscatal.5c05921

Establishing a High-Yield Bacillus subtilis-Based Cell-Free Protein Synthesis System for In Vitro Prototyping and Natural Product Biosynthesis

Xiangyang Ji, Wan-Qiu Liu, Zhiling Cao, Shuhui Huang and Jian Li

ACS Synth. Biol., 2025, 14, 1288
DOI: 10.1021/acssynbio.5c00021

Co-cultivation strategies for natural product discovery from actinomycetes: unlocking silent secondary metabolism with mycolic acid-containing bacteria

Shumpei Asamizu

World J Microbiol Biotechnol, 2025, 41
DOI: 10.1007/s11274-025-04406-7

Reaction Engineering of In Vitro Natural Product Biosynthesis: Challenges and Strategies

Elsa Sánchez‐García, Stephan Lütz and Markus Nett

ChemBioChem, 2025
DOI: 10.1002/cbic.202500571

Construction and optimization of a cell-free metabolic engineering system for lycopene synthesis

Suqing Chen, Guocui Zhu, Zhijie Wang, Bowen Fu, Mengjuan Sun, Jie Liu, Chengjia Xie and Xian Xu

Biochemical Engineering Journal, 2025, 222, 109849
DOI: 10.1016/j.bej.2025.109849

Snake venom-derived peptides as anticancer candidates: Pioneering next-generation therapies

José R. Almeida, Edgar A. Pinos-Tamayo, Bruno Mendes, Alberto A. Robles-Loaiza, Rony Abdi Syahputra, Ana Gabriela Silva Oliveira and Rosy Iara Maciel de A. Ribeiro

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, 2025, 1880, 189479
DOI: 10.1016/j.bbcan.2025.189479