Issue 10, 2019

Label-free and sensitive detection of coralyne and heparin based on target-induced G-quadruplex formation

Abstract

Herein we propose a label-free and sensitive detection method for coralyne and heparin, based on utilizing the complex of adenosine16 (A16) and coralyne to induce the formation of a G-quadruplex scaffold. In the present work, two specific oligonucleotide strands were designed. Upon addition of coralyne, the two oligonucleotide strands formed a double chain through A2–coralyne–A2 coordination, which induced the formation of an intermolecular G-quadruplex accompanied by the proximity of two G-rich sequences. An increase in system fluorescence was observed after the addition of N-methylmorpholine (NMM). In the presence of heparin, the formation of the heparin–coralyne complex caused coralyne to be removed from the A16–coralyne complex, leading to a weaker fluorescence output. At optimal concentrations, a linear coralyne response could be observed from 0.01–5 μM and the detection limit was estimated to be 5.8 nM. The detection limit of the heparin probe was as low as 1 nM, with a linear range from 1–100 nM. Overall, this study offers a sensitive and practical assay for coralyne and heparin detection.

Graphical abstract: Label-free and sensitive detection of coralyne and heparin based on target-induced G-quadruplex formation

Supplementary files

Article information

Article type
Paper
Submitted
22 一月 2019
Accepted
05 二月 2019
First published
06 二月 2019

Anal. Methods, 2019,11, 1331-1337

Label-free and sensitive detection of coralyne and heparin based on target-induced G-quadruplex formation

M. Chen, C. Ma, H. Zhao and K. Wang, Anal. Methods, 2019, 11, 1331 DOI: 10.1039/C9AY00175A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements