Issue 2, 2016

New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

Abstract

Covering: 2010 to 2015

Natural product scaffolds remain a major source and inspiration for human therapeutics. However, generation of a natural product in the post-genomic era often requires reconstruction of the corresponding biosynthetic gene cluster in a heterologous host. In the burgeoning fields of synthetic biology and metabolic engineering, a significant amount of efforts has been devoted to develop DNA assembly techniques with higher efficiency, fidelity, and modularity, and heterologous expression systems with higher productivity and yield. Here we describe recent advances in DNA assembly and host engineering and highlight their applications in natural product discovery and engineering.

Graphical abstract: New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

Article information

Article type
Highlight
Submitted
02 八月 2015
First published
09 十二月 2015

Nat. Prod. Rep., 2016,33, 174-182

Author version available

New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

Y. Luo, B. Enghiad and H. Zhao, Nat. Prod. Rep., 2016, 33, 174 DOI: 10.1039/C5NP00085H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements